Our research and innovation efforts contribute to the development of improved health for all. To this end, we work discovering and enhancing the microbial production of pharmacological ingredients, applying molecular technologies to the development of better prophylactic tools for animal health, and providing knowledge in the design of novel diagnostic devices.
Inbiotec: Instituto de biotecnología de León
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Language English
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Health
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Microbial APIs production
Microorganisms are main producers of Active Pharmacological ingredients (APIs).
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Vaccines for animal health
As a pillar of the one health concept, animal health is at the root of global health, enabling the control of classical zoonoses, emerging infectious diseases and environmental threats.
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In-vitro diagnostics
Rapid, accurate, affordable and easy-to-handle diagnostic devices are crucial for facilitating point-of-care testing and empowering individuals for self- management of health.
Related projects:
- 2018 Producción de Cannabinoides con aplicación farmacológica mediante el desarrollo de una innovadora tecnología de fermentación basada en plataformas biotecnológicas Fúngicas (RTC-2017-6173-1)
- 2018 Syntheroids: Synthetic Biology for Industrial Production of Steroids [Ref. no.: ERA-CoBioTech: ID. 48; Ministerio de Ciencia, Innovación y Universidades (PCI2018-093066)]
- 2018 AgroInnova GO CyL Estudio de complejos naturales inocuos para la salud en alubia y lúpulo (Exp.: 47/18/126/G02)
- 2017 TOPCAPI: Thoroughly Optimised Production Chassis for Advanced Pharmaceutical Ingredients (ref. no. 720793)
- 2016 TacroDrugs: Factorías celulares de Streptomyces para la producción de fármacos tacrólogos
Related publications:
- 2016 Barreiro C*, Ullán RV. DNA-synthesizing enzymes as antibacterial targets (Chapter 4). 2016. In: New weapons to control bacterial growth. Editor: Tomás G. Villa y Miguel Viñas. Springer International Publishing. Hardcover ISBN: 978-3-319-28366-1. (*Corresponding author).
- 2016 Ullán RV, Barreiro C. Bacterial protease as targets to control bacterial growth (Capítulo 6). 2016. En: New weapons to control bacterial growth. Editor: Tomás G. Villa y Miguel Viñas. Editorial: Springer International Publishing. Hardcover ISBN: 978-3-319-28366-1.
- 2015 ZEILINGER S., GARCÍA-ESTRADA C., MARTÍN J.F. (2015). Fungal secondary metabolites in the “OMICS” era. In Biosynthesis and molecular genetics of fungal secondary metabolites Vol II. (eds. S Zeilinger, J.F. Martín, C. García-Estrada and). Springer US, New York, NY 10013, USA., pp. 1-12. ISBN 978-1-4939-2530-8.
- 2015 GARCÍA-ESTRADA C., ULLÁN R.V. (2015). RNAi-mediated gene silencing in the beta-lactam producer fungi Penicillium chrysogenum and Acremonium chrysogenum. In Genetic Transformation Systems in Fungi, Vol. 2 (eds. M.A. Van den Berg and K. Maruthachalam). Springer International Publishing, Switzerland, pp.125-130. ISBN 978-3-319-10502-4.
- 2015 Domínguez-Santos R, García-Estrada C, Kosalková K, Prieto C, Santamarta I, Martín JF. PcFKH1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in Penicillium chrysogenum. Biochimie. 2015;115:162-76. Epub 2015 Jun 4. 26049046.
- 2015 Barreiro C (Ed, preface). New trends in Corynebacterium glutamicum: beyond the amino acids. 2015. , Nova Science Publishers (New York, USA). ISBN: 978-1-63483-280-9.
- 2015 Barreiro C. Methods in Proteomics applied to Corynebacterium glutamicum. 2015., New trends in Corynebacterium glutamicum: beyond the amino acids.Nova Science Publishers (New York, USA). ISBN: 978-1-63483-280-9.
- 2015 Pérez-Redondo, R.; Sola-Landa, A. & Rodríguez-García, A.Barreiro, C., ed., (2015), New Trends in Corynebacterium glutamicum: Beyond the Amino Acids., Nova Science Publishers, New York, chapter Chapter 2: Deciphering the Dynamic Transcriptome Employing Microarrays., pp. 13–44. (Book chapter)
- 2014 Balaña-Fouce R, Alvarez-Velilla R, Fernández-Prada C, García-Estrada C, Reguera RM. Trypanosomatids topoisomerase re-visited. New structural findings and role in drug discovery. Int J Parasitol Drugs Drug Resist. 2014 Aug 24;4(3):326-37. doi: 10.1016/j.ijpddr.2014.07.006. eCollection 2014 Dec. Review. 25516844.
- 2014 Cepeda-García C, Domínguez-Santos R, García-Rico RO, García-Estrada C, Cajiao A, Fierro F, Martín JF. Direct involvement of the CreA transcription factor in penicillin biosynthesis and expression of the pcbAB gene in Penicillium chrysogenum. Appl Microbiol Biotechnol. 2014 Aug;98(16):7113-24. Epub 2014 May 13. 24818689.
- 2014 GARCÍA-ESTRADA C., MARTÍN J.F. (2014) Penicillins. In Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Vol. I (eds. C. García-Estrada, S Zeilinger and J.F. Martín). Springer US, New York, NY 10013, USA., pp. 17-42. ISBN 978-1-4939-1190-5.
- 2014 DOMÍNGUEZ-SANTOS R., MARTÍN J.F., GARCÍA-ESTRADA C. (2013) Regulatory Processes Controlling the Biosynthesis of Beta-lactam Antibiotics in Penicillium chrysogenum and Acremonium chrysogenum. In Recent Developments in Biotechnology (Drug Discovery) Vol. 9 (ed. V.K. Govil). Studium Press LLC, Houston, Texas 77072, USA., Chapter 7, pp. 225-262. ISBN 1-62699-022-0.
- 2012 Martín, J.-F.; Sola-Landa, A. & Rodríguez-García, A.Gross, R. & Beier, D., ed., (2012), Two-Component Systems in Bacteria, Caister Academic Press, chapter Chapter 15 Two-component Systems in Streptomyces, pp. 315–331.
- 2012 Rodríguez-García, A.Alejandre, A. P., ed., (2012), Aplicación de la Biotecnología en la Ciencia Veterinaria, International Marketing and Communication SA, chapter Aplicaciones de la tecnologГa de microarrays o micromatrices, pp. 237–251.
- 2012 BARREIRO C., GARCÍA-ESTRADA C., MARTÍN J.F. (2012) Proteomics Methodology Applied to the Analysis of Filamentous Fungi – New Trends for an Impressive Diverse Group of Organisms. In Applications of Tandem mass Spectrometry (Ed. J. Prasain and G. L. Harn). Intech-Open Access Publisher, Rijeka, Croatia., pp. 127-160. ISBN: 978-953-307-870-0.
- 2012 MARTÍN J.F., GARCÍA-ESTRADA C., ULLÁN R.V. (2012) Genes encoding penicillin and cephalosporin biosynthesis in Acremonium chrysogenum: two separate clusters are required for cephalosporin production. In Biotechnology of fungal genes (ed. V.K. Gupta and M. Ayyachamy). Science Publishers (An Imprint of Edenbridge Ltd, British Channel Islands), Enfield, New Hampshire 03478, USA., pp. 113-138. ISBN: 978-1-57808-787-7.
- 2012 García-Estrada C. (2012) Bases moleculares de la producción de antibióticos beta-lactámicos. En Aplicación de la Biotecnología en la Ciencia Veterinaria (ed. Fundación Tomás Pascual y Pilar Gómez-Cuétara y Real Academia de Ciencias Veterinarias). IMC-SA, Madrid 28036, Spain. pp. 57-104. ISBN: 978-84-7867-202-8.
- 2011 Pérez-Pertejo, Y, Alvarez-Velilla, R, Estrada, CG, Balaña-Fouce, R, Reguera, RM, Leishmania donovani: proteasome-mediated down-regulation of methionine adenosyltransferase.Parasitology 138:1082-92 Aug 2011. 21813028
- 2011 Martín, J. F.; Sola-Landa, A.; Santos Beneit, F. & Rodríguez-García, A.Dyson, P., ed., (2011), Streptomyces: Molecular Biology and Biotechnology, Caister Academic Press, chapter Chapter 7 Network mechanisms of phosphate control of primary and secondary metabolism, pp. 137–149.
- 2011 Liras P., Santamarta I., Pérez-Redondo R. Dyson P., ed., (2011), Streptomyces: Molecular Biology and Biotechnology, Caister Academic Press, Reino Unido. Chapter 9: Clavulanic Acid and Clavams Biosynthesis and Regulation, pp. 167–178.
- 2011 GARCÍA-ESTRADA C., MARTÍN J.F. (2011) Penicillins and cephalosporins. In Comprehensive Biotechnology (ed. Moo-Young Murray). ELSEVIER, Amsterdam. vol. 3, pp. 255-268. ISBN: 978-0-444-53352-4.
- 2010 García-Estrada, C, Prada, CF, Fernández-Rubio, C, Rojo-Vázquez, F, Balaña-Fouce, R, DNA topoisomerases in apicomplexan parasites: promising targets for drug discovery.Proc. Biol. Sci. 277:1777-87 Jun 2010. 20200034
- 2010 GARCÍA-ESTRADA C., FIERRO F., MARTÍN J.F. (2010) Evolution of fungal β-lactam biosynthesis gene clusters. In Microbiology Book Series number 2: Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (Volume 1) (ed. A. Méndez-Villas). FORMATEX RESEARCH CENTER, Badajoz, pp.577-588. ISBN (13): 978-84-614-6194-3.
- 2009 Ordóñez, C, Alfonso, J, Balaña-Fouce, R, Ordóñez, D, Functional expression of a DNA-topoisomerase IB from Cryptosporidium parvum.J. Biomed. Biotechnol. 2009:837608 2009. 19644560
- 2008 Reguera, RM, Díaz-González, R, Pérez-Pertejo, Y, Balaña-Fouce, R, Characterizing the bi-subunit type IB DNA topoisomerase of Leishmania parasites; a novel scenario for drug intervention in trypanosomatids.Curr Drug Targets 9:966-78 Nov 2008. 18991609
- 2008 Diaz-González, R, Pérez-Pertejo, Y, Pommier, Y, Balaña-Fouce, R, Reguera, RM, Mutational study of the “catalytic tetrad” of DNA topoisomerase IB from the hemoflagellate Leishmania donovani: Role of Asp-353 and Asn-221 in camptothecin resistance.Biochem. Pharmacol. 76:608-19 Sep 2008. 18655776
- 2008 Balaña-Fouce, R, García-Estrada, C, Pérez-Pertejo, Y, Reguera, RM, Gene disruption of the DNA topoisomerase IB small subunit induces a non-viable phenotype in the hemoflagellate Leishmania major.BMC Microbiol. 8:113 2008. 18611247
- 2008 García-Estrada, C, Pérez-Pertejo, Y, Ordóñez, D, Balaña-Fouce, R, Reguera, RM, Characterization of the 5′ region of the Leishmania infantum LORIEN/MAT2 gene cluster and role of LORIEN flanking regions in post-transcriptional regulation.Biochimie 90:1325-36 Sep 2008. 18420039
- 2007 Reguera, RM, Pérez-Pertejo, Y, Redondo, CM, Díaz-González, R, Balaña-Fouce, R, [Type I DNA topoisomerase from protozoan pathogens as a potential target for anti-tumoral drugs].Medicina (B Aires) 67:747-57 2007. 18422072
- 2007 Díaz González, R, Pérez Pertejo, Y, Ordóñez, D, Balaña-Fouce, R, Reguera, RM, Deletion study of DNA topoisomerase IB from Leishmania donovani: searching for a minimal functional heterodimer.PLoS ONE 2:e1177 2007. 18000548
- 2007 Díaz González, R, Pérez Pertejo, Y, Redondo, CM, Pommier, Y, Balaña-Fouce, R, Reguera, RM, Structural insights on the small subunit of DNA topoisomerase I from the unicellular parasite Leishmania donovani.Biochimie 89:1517-27 Dec 2007. 17900785
- 2007 Balaña-Fouce, R, Reguera, RM, RNA interference in Trypanosoma brucei: a high-throughput engine for functional genomics in trypanosomatids?Trends Parasitol. 23:348-51 Aug 2007. 17604223
- 2007 García-Estrada, C, Pérez-Pertejo, Y, Ordóñez, D, Balaña-Fouce, R, Reguera, RM, Analysis of genetic elements regulating the methionine adenosyltransferase gene in Leishmania infantum.Gene 389:163-73 Mar 2007. 17196769
- 2007 Martínez-Valladares, M, Godio-Fernández, R, Vara-Del Río, MP, Martín, JF, Rojo-Vázquez, FA, Expression of the recombinant protein disulphide isomerase of Teladorsagia circumcincta.Parasite Immunol. 29:47-56 Jan 2007. 17187654
- 2007 GARCÍA-ESTRADA C., MARTÍN J.F. (2007) Penicillin biosynthesis and Secretion: Precursor and Enzyme Localization. In Mycotechnology: Present Status and Future Prospects (ed. Mahendra Rai). I.K. International Publishing House Pvt Ltd, New Delhi, pp.55-83. ISBN 81-89866-08-7.
- 2006 Reguera, RM, Redondo, CM, Gutierrez de Prado, R, Pérez-Pertejo, Y, Balaña-Fouce, R, DNA topoisomerase I from parasitic protozoa: a potential target for chemotherapy.Biochim. Biophys. Acta 1759:117-31. 16757380
- 2006 Pérez-Pertejo, Y, Reguera, RM, Ordóñez, D, Balaña-Fouce, R, Characterization of a methionine adenosyltransferase over-expressing strain in the trypanosomatid Leishmania donovani.Biochim. Biophys. Acta 1760:10-9 Jan 2006. 16280200
- 2005 Reguera, RM, Tekwani, BL, Balaña-Fouce, R, Polyamine transport in parasites: a potential target for new antiparasitic drug development.Comp. Biochem. Physiol. C Toxicol. Pharmacol. 140:151-64 Feb 2005. 15907761
- 2004 Pérez-Pertejo, Y, Reguera, RM, García-Estrada, C, Balaña-Fouce, R, Ordóñez, D, Mutational analysis of methionine adenosyltransferase from Leishmania donovani.Eur. J. Biochem. 271:2791-8 Jul 2004. 15206944
- 2003 PÉREZ-PERTEJO, Y., DÍAZ-GONZÁLEZ, R., GARCÍA-ESTRADA, C., REGUERA, R. M. AND BALAÑA-FOUCE, R. (2003) S-adenosylmethionine metabolism in Leishmania parasites. Recent Research Developments in Microbiology. Ed. S.G. Pandalai, Research Signpost, Vol. 7. Part II: 547-560.
- 2003 Villa, H, Pérez-Pertejo, Y, García-Estrada, C, Reguera, RM, Requena, JM, Tekwani, BL, Balaña-Fouce, R, Ordóñez, D, Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani.Eur. J. Biochem. 270:4339-47 Nov 2003. 14622299
- 2003 García-Estrada, C, Reguera, RM, Villa, H, Requena, JM, Müller, S, Pérez-Pertejo, Y, Balaña-Fouce, R, Ordóñez, D, Identification of a gene in Leishmania infantum encoding a protein that contains a SP-RING/MIZ zinc finger domain.Biochim. Biophys. Acta 1629:44-52 Oct 2003. 14522079
- 2003 Pérez-Pertejo, Y, Reguera, RM, Villa, H, García-Estrada, C, Balaña-Fouce, R, Pajares, MA, Ordóñez, D, Leishmania donovani methionine adenosyltransferase. Role of cysteine residues in the recombinant enzyme.Eur. J. Biochem. 270:28-35 Jan 2003. 12492472
- 2003 Villa, H, Otero Marcos, AR, Reguera, RM, Balaña-Fouce, R, García-Estrada, C, Pérez-Pertejo, Y, Tekwani, BL, Myler, PJ, Stuart, KD, Bjornsti, MA, Ordóñez, D, A novel active DNA topoisomerase I in Leishmania donovani.J. Biol. Chem. 278:3521-6 Feb 2003. 12444094
- 2002 Reguera, RM, Balaña-Fouce, R, Pérez-Pertejo, Y, Fernández, FJ, García-Estrada, C, Cubría, JC, Ordóñez, C, Ordóñez, D, Cloning expression and characterization of methionine adenosyltransferase in Leishmania infantum promastigotes.J. Biol. Chem. 277:3158-67 Feb 2002. 11698393
- 1999 Reguera, RM, Pérez-Pertejo, Y, Ordóñez, C, Cubría, JC, Tekwani, BL, Balaña-Fouce, R, Ordóñez, D, S-adenosylmethionine synthesis in Leishmania infantum promastigotes.Cell Biol. Int. 23:579-83 1999. 10704242
- 1999 Cubría, JC, Ordóñez, C, Reguera, RM, Tekwani, BL, Balaña-Fouce, R, Ordóñez, D, Early alterations of polyamine metabolism induced after acute administration of clenbuterol in mouse heart.Life Sci. 64:1739-52 1999. 10353628
- 1998 Cepero, M, Cubría, JC, Reguera, R, Balaña-Fouce, R, Ordóñez, C, Ordóñez, D, Plasma and muscle polyamine levels in aerobically exercised rats treated with salbutamol.J. Pharm. Pharmacol. 50:1059-64 Sep 1998. 9811168
- 1998 Johnson, R, Cubría, JC, Reguera, RM, Balaña-Fouce, R, Ordóñez, D, Interaction of cationic diamidines with Leishmania infantum DNA.Biol. Chem. 379:925-30 Jul 1998. 9705158
- 1998 Balaña-Fouce, R, Reguera, RM, Cubría, JC, Ordóñez, D, The pharmacology of leishmaniasis.Gen. Pharmacol. 30:435-43 Apr 1998. 9580315
- 1996 Calonge, M, Cubría, JC, Balaña-Fouce, R, Ordóñez, D, Putrescine active uptake system in the Trypanosomatid Crithidia fasciculata.Biol. Chem. Hoppe-Seyler 377:233-8 Apr 1996. 8737988
- 1996 Calonge M, Bayoumi AE, Cubría JC, Balaña-Fouce R and Ordóñez D. Effects of cationics diamidines on polyamine metabolis in the Trypanomatid Crithidia fasciculate. Life Sci.1996; 59 (12): 191. 8809218